Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study (2024)

van Veldhoven, K., Keski-Rahkonen, P., Barupal, D. K., Villanueva, C. M., Font-Ribera, L., Scalbert, A., Bodinier, B., Grimalt, J. O., Zwiener, C., Vlaanderen, J., Portengen, L., Vermeulen, R., Vineis, P., Chadeau-Hyam, M., & Kogevinas, M. (2018). Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study. Environmental International, 111, 60-70. https://doi.org/10.1016/j.envint.2017.11.017

van Veldhoven, Karin ; Keski-Rahkonen, Pekka ; Barupal, Dinesh K et al. / Effects of exposure to water disinfection by-products in a swimming pool : A metabolome-wide association study. In: Environmental International. 2018 ; Vol. 111. pp. 60-70.

@article{c036a22fc69446a29cba75a4207342de,

title = "Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study",

abstract = "BACKGROUND: Exposure to disinfection by-products (DBPs) in drinking water and chlorinated swimming pools are associated with adverse health outcomes, but biological mechanisms remain poorly understood.OBJECTIVES: Evaluate short-term changes in metabolic profiles in response to DBP exposure while swimming in a chlorinated pool.MATERIALS AND METHODS: The PISCINA-II study (EXPOsOMICS project) includes 60 volunteers swimming 40min in an indoor pool. Levels of most common DBPs were measured in water and in exhaled breath before and after swimming. Blood samples, collected before and 2h after swimming, were used for metabolic profiling by liquid-chromatography coupled to high-resolution mass-spectrometry. Metabolome-wide association between DBP exposures and each metabolic feature was evaluated using multivariate normal (MVN) models. Sensitivity analyses and compound annotation were conducted.RESULTS: Exposure levels of all DBPs in exhaled breath were higher after the experiment. A total of 6,471 metabolic features were detected and 293 features were associated with at least one DBP in exhaled breath following Bonferroni correction. A total of 333 metabolic features were associated to at least one DBP measured in water or urine. Uptake of DBPs and physical activity were strongly correlated and mutual adjustment reduced the number of statistically significant associations. From the 293 features, 20 could be identified corresponding to 13 metabolites including compounds in the tryptophan metabolism pathway.CONCLUSION: Our study identified numerous molecular changes following a swim in a chlorinated pool. While we could not explicitly evaluate which experiment-related factors induced these associations, molecular characterization highlighted metabolic features associated with exposure changes during swimming.",

keywords = "Disinfection by-products, DBPs, Metabolome, LC-MS, Blood, Exposome",

author = "{van Veldhoven}, Karin and Pekka Keski-Rahkonen and Barupal, {Dinesh K} and Villanueva, {Cristina M.} and Laia Font-Ribera and Augustin Scalbert and Barbara Bodinier and Grimalt, {Joan O} and Christian Zwiener and Jelle Vlaanderen and L{\"u}tzen Portengen and Roel Vermeulen and Paolo Vineis and Marc Chadeau-Hyam and Manolis Kogevinas",

note = "Copyright {\textcopyright} 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.",

year = "2018",

month = feb,

doi = "10.1016/j.envint.2017.11.017",

language = "English",

volume = "111",

pages = "60--70",

journal = "Environmental International",

issn = "0160-4120",

publisher = "Elsevier Limited",

}

van Veldhoven, K, Keski-Rahkonen, P, Barupal, DK, Villanueva, CM, Font-Ribera, L, Scalbert, A, Bodinier, B, Grimalt, JO, Zwiener, C, Vlaanderen, J, Portengen, L, Vermeulen, R, Vineis, P, Chadeau-Hyam, M & Kogevinas, M 2018, 'Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study', Environmental International, vol. 111, pp. 60-70. https://doi.org/10.1016/j.envint.2017.11.017

Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study. / van Veldhoven, Karin; Keski-Rahkonen, Pekka; Barupal, Dinesh K et al.
In: Environmental International, Vol. 111, 02.2018, p. 60-70.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Effects of exposure to water disinfection by-products in a swimming pool

T2 - A metabolome-wide association study

AU - van Veldhoven, Karin

AU - Keski-Rahkonen, Pekka

AU - Barupal, Dinesh K

AU - Villanueva, Cristina M.

AU - Font-Ribera, Laia

AU - Scalbert, Augustin

AU - Bodinier, Barbara

AU - Grimalt, Joan O

AU - Zwiener, Christian

AU - Vlaanderen, Jelle

AU - Portengen, Lützen

AU - Vermeulen, Roel

AU - Vineis, Paolo

AU - Chadeau-Hyam, Marc

AU - Kogevinas, Manolis

N1 - Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2018/2

Y1 - 2018/2

N2 - BACKGROUND: Exposure to disinfection by-products (DBPs) in drinking water and chlorinated swimming pools are associated with adverse health outcomes, but biological mechanisms remain poorly understood.OBJECTIVES: Evaluate short-term changes in metabolic profiles in response to DBP exposure while swimming in a chlorinated pool.MATERIALS AND METHODS: The PISCINA-II study (EXPOsOMICS project) includes 60 volunteers swimming 40min in an indoor pool. Levels of most common DBPs were measured in water and in exhaled breath before and after swimming. Blood samples, collected before and 2h after swimming, were used for metabolic profiling by liquid-chromatography coupled to high-resolution mass-spectrometry. Metabolome-wide association between DBP exposures and each metabolic feature was evaluated using multivariate normal (MVN) models. Sensitivity analyses and compound annotation were conducted.RESULTS: Exposure levels of all DBPs in exhaled breath were higher after the experiment. A total of 6,471 metabolic features were detected and 293 features were associated with at least one DBP in exhaled breath following Bonferroni correction. A total of 333 metabolic features were associated to at least one DBP measured in water or urine. Uptake of DBPs and physical activity were strongly correlated and mutual adjustment reduced the number of statistically significant associations. From the 293 features, 20 could be identified corresponding to 13 metabolites including compounds in the tryptophan metabolism pathway.CONCLUSION: Our study identified numerous molecular changes following a swim in a chlorinated pool. While we could not explicitly evaluate which experiment-related factors induced these associations, molecular characterization highlighted metabolic features associated with exposure changes during swimming.

AB - BACKGROUND: Exposure to disinfection by-products (DBPs) in drinking water and chlorinated swimming pools are associated with adverse health outcomes, but biological mechanisms remain poorly understood.OBJECTIVES: Evaluate short-term changes in metabolic profiles in response to DBP exposure while swimming in a chlorinated pool.MATERIALS AND METHODS: The PISCINA-II study (EXPOsOMICS project) includes 60 volunteers swimming 40min in an indoor pool. Levels of most common DBPs were measured in water and in exhaled breath before and after swimming. Blood samples, collected before and 2h after swimming, were used for metabolic profiling by liquid-chromatography coupled to high-resolution mass-spectrometry. Metabolome-wide association between DBP exposures and each metabolic feature was evaluated using multivariate normal (MVN) models. Sensitivity analyses and compound annotation were conducted.RESULTS: Exposure levels of all DBPs in exhaled breath were higher after the experiment. A total of 6,471 metabolic features were detected and 293 features were associated with at least one DBP in exhaled breath following Bonferroni correction. A total of 333 metabolic features were associated to at least one DBP measured in water or urine. Uptake of DBPs and physical activity were strongly correlated and mutual adjustment reduced the number of statistically significant associations. From the 293 features, 20 could be identified corresponding to 13 metabolites including compounds in the tryptophan metabolism pathway.CONCLUSION: Our study identified numerous molecular changes following a swim in a chlorinated pool. While we could not explicitly evaluate which experiment-related factors induced these associations, molecular characterization highlighted metabolic features associated with exposure changes during swimming.

KW - Disinfection by-products

KW - DBPs

KW - Metabolome

KW - LC-MS

KW - Blood

KW - Exposome

U2 - 10.1016/j.envint.2017.11.017

DO - 10.1016/j.envint.2017.11.017

M3 - Article

C2 - 29179034

SN - 0160-4120

VL - 111

SP - 60

EP - 70

JO - Environmental International

JF - Environmental International

ER -

van Veldhoven K, Keski-Rahkonen P, Barupal DK, Villanueva CM, Font-Ribera L, Scalbert A et al. Effects of exposure to water disinfection by-products in a swimming pool: A metabolome-wide association study. Environmental International. 2018 Feb;111:60-70. doi: 10.1016/j.envint.2017.11.017